Pair Name	Piperlongumine, Oxaliplatin
Disease Info	[ICD-11: 2B72]	Gastric cancer		Investigative
Biological Phenomena	Induction-->DNA damage
Gene Regulation	Up-regulation	Expression	H2AX	hsa3014
	Up-regulation	Phosphorylation	MAPK14	hsa1432
	Up-regulation	Phosphorylation	MAPK8	hsa5599
In Vitro Model	SGC-7901	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_0520
	BGC-823	Human papillomavirus-related cervical adenocarcinoma	Homo sapiens (Human)	CVCL_3360
In Vivo Model	HCT116 cells (5×10⁶ cells in 100 μl PBS) were harvested and injected subcutaneously into the right flanks of the mice. Next, the mice were divided into four experimental groups on randomization and blinding, with four mice in each group.
Result	Piperlongumine significantly enhanced oxaliplatin-induced growth inhibition of these cells and that TrxR1 activity is involved in their synergistic effect both in vitro and in vivo. These data suggest that PL and oxaliplatin combination treatment of gastric cancer may be more effective than oxaliplatin alone.

Pair Name	Piperlongumine, Oxaliplatin
Disease Info	[ICD-11: 2B90]	Colon cancer		Investigative
Biological Phenomena	Induction-->Oxidative Stress
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Activity	CASP3	hsa836
In Vitro Model	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	LoVo	Colon adenocarcinoma	Homo sapiens (Human)	CVCL_0399
In Vivo Model	For a xenograft model, HCT-116 cells (1×10⁷ in 150 uL PBS) were subcutaneously injected into the right flank of five-week-old female athymic BALB/c nu/nu mice.
Result	This study provides a novel combination therapy for colorectal cancer, and reveals that manipulating ROS production might constitute an effective tool for developing novel treatments in colorectal cancer.